Localization of enzymes involved in the synthesis of cell wall matrix polysaccharides within the Golgi apparatus in plants The Golgi apparatus in plants contains at least three separate sub-compartments, the cis, medial and trans-Golgi compartments. The aim of this project is to determine how the enzymes involved in the synthesis of cell wall matrix polysaccharides are organized within this apparatus. This work established that the methyltransferases formed both EDTA-soluble and EDTA-insoluble methylated polysaccharides in the Golgi apparatus, chiefly in the cis and medial sub-compartments. The results indicated that the EDTA-soluble and -insoluble products consisted mainly of methyl-esterified pectins and 4-0-methyl glucuronoxylan, respectively. This led to the conclusion that both pectin methyltransferase and glucuronoxylan methyltransferase are located in the cis and medial Golgi membranes. E. Baydoun. (Supported by URB.) Comparative evaluation of chromatographic and immunoassay procedures for pesticide analysis The focus of this project is on the compound DDE which is a persistent and toxic metabolite of DDT. It tends to become biomagnified and deposits particularly in the adipose tissue of humans and animals. Recent studies detected DDE residues in concentration that increased with age in the adipose tissue of human subjects. The immunoassay used is a polycloncal antibody based assay where the antibody is coated on microwell plates. DDE in the test material is allowed to compete with an enzyme labelled pesticide for antibody binding. Unbound enzyme labelled pesticide is removed by washing with water and bound enzyme labelled pesticide is activated by adding a mixture of substrate-chromogen which produces a coloured product. DDE in the sample is quantified by comparing colour development in the sample wells with colour development in wells that correspond to the standard curve. S. Dagher. (Supported by International Atomic Energy Agency.) Molecular characterization of enterococcal species recovered from various sources Epidemiological studies on Enterococci have been limited by lack of convenient methods for comparing strains. Among the methods used to characterize the isolates are the total plasmid content, analysis of chromosomal DNA by restriction endonuclease digestion and pulse field gel electrophoresis. Due to some difficulties and inconsistency encountered in some of the methods, arbitrary primer polymerase chain reaction (AP-PCR) and pulse field gel electrophoresis were chosen to characterize the various enterococcal species previously collected from clinical specimens and from the gastrointestinal tract of man. AP-PCR was done in a pilot study on ten clinical E. faecalis isolates. Data obtained have shown several patterns which denotes that heterogeneity exist among the isolates. Additional work is underway to subtype all the enterococcal isolates, which may determine the existence of a link between the isolates recovered as normal flora and those obtained from clinical specimens. H. Harakeh and G. Matar. (Supported by URB.) Enterococcal species recovered from clinical specimens and the gastrointestinal tract of man In an attempt to study the incidence of various enterococcal species encountered in clinical specimens, a total of 72 isolates were collected as gram positive cocci-coccobacilli. A total of 67 isolates (93.1%) were identified as Enterococci (59 E. faecalis, 7 E. faecium, and 1 E. durans) and only 5 (6.9%) ýwere shown to belong to other genera. All isolates proved to be susceptible to vancomycin and teicoplanin; 12.5% and 87.5% of the isolates were resistant to ampicillin and gentamicin respectively. A total of 49 rectal swabs were obtained from hospitalized patients with non-gastrointestinal infections. Forty-two Enterococci were recovered and had the following species distribution: faecalis 24 (57.1%) faecium 8 (19.0), cassiliflavis 4 (9.5%), avium 4 (95%) and durans 2 (4.7%). All Enterococci except E. faecium proved to be susceptible to ampicillin, gentamicin, vancomycin and teicoplanin. Three of the 8 E. faecium were only susceptible to gentamicin teicoplanin and vancomycin. H. Harakeh and M. Uwaydah. (Supported by URB.) Effects of various analgesic and anti-inflammatory drugs on endotoxin-induced hyperalgesia in rats and mice A new model of endotoxin (ET) induced hyperalgesia has been used to test the effects of four classes of drugs in rats and mice. Hyperalgesia was assessed by paw pressure (PP), Hot Plate (HP) and Tail Flick (TF) tests. Each drug was injected intraperitoneally 24 and 12 h before ET injection. At the dosages used, acetaminophen and dexamethasone were the most effective on TF hyperalgesia, while indomethacin and morphine produced their main effect on TF hyperalgesia. The four drugs were about equally effective in reversing HP hyperalgesia. We conclude that ET hyperalgesia is mediated by both prostaglandin-sensitive and prostaglandin-independent mechanisms. S. A. Kanaan, B. Safieh-Garabedian, J. J. Haddad, S.F. Atweh, A.M. Abdelnoor, S.J. Jabbur and N.E. Saade. (Supported by URB; Diana Tamari Sabbagh Fund; LNCSR). The effect of thymulin on Leishmania major-induced inflammation in Balb/c mice Leishmaniasis is a well known protozoan infection affecting mammalian hosts, ranging in severity from a self-healing cutaneous lesion to a total disseminated disease. The outcome of experimental infection by L. major in inbred strains of mice is influenced by macrophages (the primary site of infection), together with T lymphocytes subsets and production of cytokines. Various immunological means were utilized to activate the infected macrophages, and thus limit the infection. Thymulin, one of the thymic hormones, has been shown to be an important immunomodulator of T lymphocytes in the expression of their markers and the production of cytokines. In this study, thymulin has been shown to reduce, ina dose dependent manner, the hyperalgesia induced by intraplantar injection of L. major parasites in Balb/c mice. We are currently investigating the effects of thymulin on the pro-inflammtory cytokines Tumor Necrosis Factor (TNF) a and IL-1b in the L. major infected mice. S.A. Kanaan, M. Karam, H. El-Khansa*, A. Jurjus and B. Safieh-Garabedian. (Supported by URB.) Interleukin (IL)-10 reduces the endotoxin (ET)-induced peripheral hyperalgesia in Balb/c mice We report that intraperitoneal injection of IL-10 (Human rDNA) significantly reduces the ET-induced peripheral hyperalgesia in Balb/c mice. Different groups of mice were injected (i.p.) with various doses of IL-10 in 100 m l saline, followed 30 min later by intraplantar ET injection. One group of mice received ET injections only and control animals received the same volume of saline injection only. All animals were subjected to thermal and mechanical hyperalgesia. IL-10 significantly reduced, and in a dose dependent manner, mechanical and thermal hyperalgesia. At 7.5 m g, IL-10 increases the pain threshold latencies of PP (from 62.6 with ETto 82%), HP (from 57% with ET to 90%) and TF (from 76% with ET to 90%) as compared to their control values. It is known that IL-10 can decrease inflammation and hyperalgesia through inhibitory action on Tumor Necrosis Factor and IL-1b and we are currently investigating its effects on both of these cytokines. S.A. Kanaan, S. Poole*, N.E. Saade, S.J. Jabbur and B. Safieh-Garabedian. (Supported by URB. Survey, biology and resource utilization of the flower-head infesting fruit flies (Tephritidae) on Asteraceae plants, in Lebanon The family Tephritidae comprises 4,000 species of fruit-infesting and non-fruit infesting fruit flies, distributed throughout the world. The non-frugivorous tephritids infest mostly flower heads of the Asteraceae. Very little is known about the identity, host range, and distribution of flower-head infesting tephritids in Lebanon. A survey of the flower-head infesting tephritids was started in Lebanon in the spring of 1995. Flower-head samples were collected from different regions in Lebanon and placed in insectary cages to monitor the emergence of fruit flies. Out of 20 plant species belonging to the Asteraceae, 14 yielded fruit flies. The fruit flies that were reared belonged to 15 species, 12 of which are reported for the first time to occur in Lebanon. K. M. Knio. (Supported by URB.) Effect of epinephrine on the mucosal uptake of phenylalanine in the rat jejunum Although adrenergic agonists and antagonists have found extensive clinical use, their effects on the intestinal absorption of amino acids have been rarely investigated. This work attempts to study the effect of epinephrine on the mucosal uptake of phenylalanine in the rat jejunum using an in situ double perfusion technique that allows the intestine and its vasculature to be perfused simultaneously and respectively with the amino acid and the hormone and its antagonists. Epinephrine reduced, by around 30%, the mucosal transport of phenylalanine by activating a1 , a2 and b adrenergic receptors. The effect of the latter was not apparent however, until the a2 receptors were blocked. S. Kreydiyyeh. (Supported by URB.) Regulatory elements in the promoter region of Oncoprotein 18 The Op18 gene encodes a cytosolic phosphoprotein that is induced in normal lymphocytes following mitogenic stimulation, and is implicated in cell cycle progression. Studies of the Op 18 gene are of particular interest because of its proposed role in signal transduction and because of its high expression in acute leukemia cells. The increase in the amount of Op18 phenotype is due to an increase in the transcription of Op18 gene, in the absence of gene amplification or rearrangement. The aim of the project is to identify, using gel retardation and footprinting analysis, the elements that regulate the transcription of this gene. Our data show the presence of several Sp1 recognition sequences as well as four novel protein binding sites in the promoter and intron I of Op18 gene. Moreover, our findings indicate that high level expression of Op18 in actively proliferating cells correlates with enhanced binding of nuclear factors to specific sites in the Op18 promoter/intron I region. R. Melhem and S. Hanash.* (Supported by URB; National Institute of Health CA26803 and CA32146.) Quantitative analysis of Op18 phosphorylation in childhood acute leukemia Oncoprotein 18 (Op18) is a major cytosolic phosphoprotein constituent of leukemia cells. There is cumulative evidence that suggests a role of Op18 in integrating signals from diverse pathways involved in cell growth. Op18 phosphorylation is induced with proliferation in a variety of cell types, and is essential for cell progression. The aim of this study is to analyze the level of Op18 and its major phosphorylated forms in acute leukemia samples by means of quantitative two-dimensional polyacrylamide gel electrophoresis. Our data indicate that Op18 phosphorylation is significantly correlated with the white blood count at the time of diagnosis, and with a high percentage of cells in the S phase. These findings suggest that strategies to inhibit Op18 expression or phosphorylation may be effective in inhibiting leukemia cell proliferation. R. Melhem and S. Hanash.* (Supported by PHS CA32146.) Antitumor-promoting effects of an oligomeric proanthocyanidin extracted from Loblolly pine bark in skin, lung and colon One objective of this project is to determine whether Loblolly Pine Bark Condensed Tannin (LPB-CT) can inhibit the tumor-promoting activities of various non-TPA type agents, such as UVB light, tobacco-specific nitrosamine (TSN) and 1,2-dimethyl hydrazine. Another goal is to characterize the anticancer effects of LPB-CT administered by diverse means, such as when supplied in drinking water, and tested in different protocols and animal tumor systems, such as skin, lung and colon. Demonstrating that the systemic effects of LPB-CT can inhibit the mechanisms of tumorigenesis in tissues and organs distant from the site of administration is a prerequisite to further mass-scale testing of synthetic or purified polyflavanoids for their potential in cancer therapy and/or prevention. H.U. Gali-Muhtasib. (Supported by Third World Academy of Sciences.) Protection against ultraviolet B radiation-induced photocarcinogenesis in hairless mice by tannic acid Topical application of the hydrolyzable tannin (HT) tannic acid was tested for its ability to inhibit the induction of ornithine decarboxylase (ODC) activity linked to skin tumor promotion by Ultraviolet B (UVB) radiation. The peak of ODC induction observed 30h after a single UVB radiation shifts to an earlier time of 5h after 2UVB treatments repeated at 24h interval. The peak of ODC induction was also found to increase 2.5-fold with a second UVB treatment. ODC activity is even induced 1h after such multiple UVB treatment. When applied 10 min before each UVB treatment, tannic acid was found to inhibit the ODC response observed at 30h after a single UVB treatment or at 5h after 2UVB treatments by at least 50-85% in a dose-dependent manner. These results suggest that tannins may be useful against the inflammatory responses associated with the exposure of skin to the tumor-promoting effects of environmental carcinogens. H.U. Gali-Muhtasib. (Supported by URB.) The effects of single dose and chronic UVB exposure on DNA synthesis in hairless mice Ultraviolet B (UVB) radiation, a well-known skin tumor promoter, was tested for its ability to induce DNA synthesis, a biochemical marker linked to the tumor promotion stage of mouse skin carcinogenesis. Treatments with UVB were found to induce DNA synthesis in a dose-dependent manner with the maximum stimulation occurring at a dose of 200-400mJ/cm2 UVB. Following an optimal UVB dose (200mJ/cm2), DNA synthesis was found to follow a defined time-course with an early period of inhibition observed at 8h. This inhibitory period observed with UVB radiation is similar to that observed following treatment with TPA. However, the two peaks of stimulations of DNA synthesis observed at h and 32h with TPA treatment were not observed following UVB radiation. Rather, a single peak of increased DNA synthesis was observed at 48h following UVB radiation. Future experiments will determine whether the UVB-induced DNA response can be altered by chronic UVB treatments. H.U. Gali-Muhtasib. (Supported by LNCSR.) A survey of Lebanese reptiles and and amphibians This study involves a survey of the Lebanese reptile and amphibian species; their geographic distribution in addition to ecological studies of these species. Part of the work will be presented at the Third World Congress of Herpetology, Prague, 2-10 August, 1997, in the form of two presentations. The first presentation is “The male sexual cycle of Lacerta media in Mount Lebanon compared with that of Lacerta laevis.” The second is entitled “The distribution and ecology of Acanthodactylus schreiberi in the south of Lebanon” and is intended for a poster. S. Hraoui-Bloquet*, M. Sabeh* and R.A. Sadek. (Supported by LNCSR.) Morphological and biochemical variation in the Lebanese populations of the common Hardun, Agama (Stellio) stellio The lizard species, Laudakia (Agama) stellio, commonly known as the hardun, is distributed throught Lebanese territory, occupying various types of habitats and exploiting several substrate types. Populations from various parts of the country are sampled to investigate the extent of morphological and biochemical variation within and between the various populations. The work is still in progress. R. A. Sadek and E. Baydoun. (Supported by URB.) Ecological studies of three high altitude lizard species in the mountains of Lebanon Three lizard species found at high altitudes were studied, especially in the mountains of Sannine and Barouk, northeast and southeast of Beirut respectively. One of the species, Lacerta fraasi, is endemic to Lebanon. The other two lizards are Lacerta laevis cf kulzeri and Mabuya vittata. The study involved determining the geographic distribution of the three species, in addition to the study of their habitat use, thermal relations and feeding ecology. Part of the work will be presented at the Third World Congress of Herpetology, Prague, 2-10 August, 1997 as “Ecological and morphological comparisons between Lacerta species living at different altitudes in Lebanon.” R. Sadek and H. Said. Intraplantar low dose of thymulin injection results in localized hyperalgesia: involvement of interleukin-1b , nerve growth factor and prostaglandin-E2 The effect of various doses of intraplantar thymulin injection on nociceptive thresholds in the hind paw of rats, was assessed using different pain tests. Also the upregulation of interleukin-1b (IL-1b ) and nerve growth factor (NGF) in the injected paw was determined by ELISA. The effect of both dexamethsone and indomethacin was investigated on thymulin-induced hyperalgesia. In addition, the effect of interleukin-1 receptor antagonist (IL-1ra), polyclonal anti-NGF antiserum and the tripeptide lys-D-pro-val (known to antagonize IL-1b and PGE2-induced hyperalgesia) was also studied. Our results will help us understand the role of cytokines and neurotrophic factors in thymulin-induced localized hyperalgesia. B. Safieh-Garabedian, S.A. Kanaan, R.H. Jalakhian, S.J. Jabbur and N.E. Saade. (Supported by URB; Diana Tamari Sabbagh Fund.) Involvement of interleukin-1b , nerve growth factor and prostaglandin-E2 in endotoxin-induced localized inflammatory hyperalgesia The purpose of this study was to investiagate the mechanisms involved in the endotoxin model of localized inflammatory hyperalgesia. For this purpose, we determined the role of proinflammatory mediators (interleukin-1b , nerve growth factor and prostaglandin-E2) by measuring the level of IL-1b and NGF. We also characterized the effect of antagonists to cytokines, nerve growth factor and prostaglandin-E2 on endotoxin-induced inflammatory hyperalgesia. B. Safieh-Garabedian, S.A. Kanaan, J. J. Haddad, P. Abou Jaoude, S.J. Jabbur and N.E. Saade. (Supported by URB; Diana Tamari Sabbagh Fund.) Characterization of ECM-degrading proteinases and their inhibitors in endotoxin-mediated local inflammation It is known that extracellular matrix (ECM) degrading proteinases are involved in the process of tissue development, wound healing, tumour invasion, and chronic inflammatory disorders such as rheumatoid arthritis. As far as we are aware there are few, if any, reports today that describe the regulation of ECM-degrading proteinases and their inhibitors in short term acute and completely reversible local inflammations. The goal of this project is to identify the ECM-degrading proteinases and their inhibitors that are involved in mediating tissue remodelling during endotoxin mediated inflammation. CD-1 mice will be used as the model animal. The temporal pattern of expression of ECM-degrading proteinases was correlated with the onset of inflammation at the site of endotoxin injection and its regression to normal non-inflamed tissue. The developmental regulation of these ECM-degrading proteinases in response to immunomodulatory agents known to have anti-inflammatory characteristics such as thymulin and Zn in addition to others (cytokines) was also investigated. R. Talhouk and B. Safieh-Garabedian. (Supported by URB.) Prenatal diagnosis and molecular screening for b -Thalassaemia in Lebanon using the ARMS-PCR technique The spectrum of mutations causing b -thalassaemia in Lebanon was previously determined in a 1987 study; however, because of the ongoing civil war at the time, the sample studied was not representative of the Lebanese population, since it was limited both geographically and religiously. A reassessment of the molecular basis of b -thalassaemia in Lebanon is presented in this study. A larger sample of chromosomes (110 chromosomes) was screened for more than 7 different mutations previously reported in Lebanon, using the amplification refractory mutation system (ARMS) analysis. The DNA were collected from the various religious groups found in Lebanon and had different geographic origins. Analysis of the pattern of mutations and their frequencies reveal interesting observations regarding the distribution and frequency of the mutations in Lebanon and gives a preliminary impression of a region- and a religion-specific mutational clustering. T. Talhouk and L. Zahed. (Supported by LNCSR.) Characterization of the interplay between hyperalgesia and ECM remodelling endotoxin mediated local and reversible acute inflammation The aim of this project is to identify endogenous growth factors
that modulate the development of the mammary gland in Awassi sheep. The
proposal focused on examining the regulation of growth promoting activities
in mammary secretions from different developmental stages of gestation
(pregnancy). Ongoing work will focus on characterizing the growth activities
detected. R. Talhouk. (Supported by LNCSR and Third World Academy
of Sciences.)
Abdo, R. A., Araj, G. F. and Talhouk, R. S., Methicillin resistant Staphylococcus aureus: Disease spectrum, biological characteristics, resistant mechanisms, and typing methods. Lebanese Medical Journal, 44, 21-30, 1996. Barbour, E.K., Abi Ghanem, D., Hamadeh, S., Eid, A., Talhouk, R. and Hilan, C.*, Characterization of non-specificity in herbal immunopotentiators of the cell-mediated and humoral immune systems of chickens. Journal of American Holisitic Veterinary Medical Association, 15, 5-7, 1996. Castonguary, A.*, Gali-Muhtasib, H.U., Perchellet, E.M.*, Gao, X.M.*, Boukharta, M.*, Jalbert, G.*, Okuda, T.*, Yoshida, T.*, Hatano, T.* and Perchellet, J.P.*, Antitumorigenic and antipromoting activities of ellagic acid, ellagitannins and oligomeric anthocyanin and procyanidin. International Journal of Oncology, 10, 367-373, 1997. Daouk, R., Dagher, S. and Sattout, E., Antifungal activity of the essential oil of Origanum syriacum L. Journal of Food Protection, 58 (10), 1147-1149, 1995. Hraoui-Bloquet, S., Sabeh, M. and Sadek, R., La presence du triton Triturus vittatus (Gray 1835) amphibien urodele au Liban. Lebanese Scientific Research Reports, 2 (1), 15-22, 1997. Kanaa, S.A., Saade, N.E., Haddad, J.J., Abdelnoor, A.M., Atweh, S.F., Jabbur, S.J. and Safieh-Garabedian, B., Endotoxin-induced local inflammation and hyperalgesia in rats and mice: A new model for inflammatory pain. Pain, 66, 373-379, 1996. Knio, K.M., Goeden, R.D.* and Headrick, D.H.*, Descriptions of immature stages of Trupanea nigricornis and T. bisetosa (Diptera: Tephritidae) from Southern California. Annals of the Entomological Society of America, 89 (1), 1-11, 1996. Knio, K.M., Goeden, R.D.*, Headrick, D.H.*, Comparative biologies of the cryptic, sympatric species, Trupanea bisetosa and T. nigricornis (Diptera: Tephritidae) in Southern California. Annals of the Entomological Society of America, 89 (2), 252-260, 1996. Kreydiyyeh, S.I., Inhibitors in tea of intestinal absorption of phenylalanine in rats. Comparative Biochemistry and Physiology, 113, 67-71, 1966. Kreydiyyeh, S.I., El-Kasti, M.M. and Bikhazi, A.B., A possible mechanism for the insulin stimulation of alanine uptake by isolated rat hepatocytes. Comparative Biochemistry and Physiology, 116C (3), 245-253, 1997. Khurana, S.*, Kreydiyyeh, S.I., Aronzon, A.*, Hoogerwef, W.A.*, Rhee, S.G.*, Donowitz, M.* and Cohen, M.E.*, Asymmetric signal trans-duction in polarized ileal Na+absorbing cell: carbachol activates brush-border but not basolateral-membrane PIP2-PLC and translocates PLC-y1 only to the bruhs border membrane. Biochemical Journal, 313, 509-518, 1996. Perchellet, J.P.*, Perchellet, E.M.*, Gali-Muhtasib, H.U. and Gao, X.M.*, Oxidant stress and multistage skin carcinogenesis. In Skin Cancer: Mechanisms and Human Relevance, H. Mukhtar (ed.), 145-180. Boca Raton, FL.: CRC Press, 1995. Perchellet, E.M.*, Gali-Muhtasib, H.U., Makkar, H.P.S.* and Perchellet, J.P.* Ability of tannins extracted from various tree leaves to inhibit the biomarkers of tumor promotion in mouse skin in vivo. International Journal of Oncology, 9, 801-809, 1996. Safieh-Garabedian, B., Poole, S.*, Allchorne, A.*, Winter, J.* and Woolf, C.J.*, Contribution of interleukin-1b to the inflammation-induced increase in nerve growth factor levels and inflammatory hyperalgesia. British Journal of Pharmacology, 115, 1265-1275, 1995. Safieh-Garabedian, B., Poole, S.*, Kanaan, S.A., Saade, N.E. and Woolf, C.J.*, Zinc reduces the hyperalgesia and the upregulation of NGF and IL-1B produced by peripheral inflammation in the rat. Neuropharmacology, 35, 599-603, 1996. Safieh-Garabedian, B., Jalakhian, R.H., Saade, N.E., Haddad, J.J., Jabbur, S.J. and Kanaan, S.A., Thymulin reduces hyperalgesia induced by peripheral endotoxin injection in rats and mice. Brain Research, 717, 179-183, 1996. Safieh-Garabedian, B., Kanaan, S.A., Jalakhian, R.H., Poole, S.*, Jabbur, J.J. and Saade, N.E., Hyperalgesia induced by low does of thymulin injections: Possible involvement of prostaglandin-E2. Journal of Neuroimmunology, 73, 162-168, 1997. Saleh, M.A., Zahed, L.F. and Talhouk, R.S., The molecular basis of b -Thalassaemia in Lebanon and its neighbouring countries. Lebanese Medical Journal, 44, 275-279, 1996. Sympson, C.*, Talhouk, R.S., Bissell, M.J.* and Werb, Z.*, The role of metallo-proteinases and their inhibitors in regulating mammary epithelial morphology and function in vivo. Perspectives in Drug Discovery and Design, 2 (3), 401-411, 1995. Talhouk, R.S., Abdo, R.A. and Saa’d, A., Lactose intolerance: Diagnosis and dietary treatment with milk substitutes. Lebanese Medical Journal, 44, 36-40, 1996. Talhouk, R.S., Neiswander, R.L.* and Schanbacher, F.L.*, Developmental regulation and partial characterization of growth factors in bovine mammary secretions. Journal of Reproduction and Fertility, 106, 221-230, 1996. Toufeili, I. and Baydoun, E. Some selected physiochemical properties of buffalo gourd (Cucurbita foetidissima HBK) root starch. Starch-Stä rke, 47, 413-415, 1995. Welter, J.F.*, Gali-Muhtasib, H.U., Crish, J.* and Eckert,
R.L.* Regulation of human involucrin promoter activity by POU domain proteins.
Journal
of Biological Chemistry, 271, 14727-14733, 1966.
ABSTRACTS, PRESENTATIONS AND PROCEEDINGS Abdo, R., Araj, G.F. and Talhouk, R.S., Methicillin resistant Staphylococcus aureus: Disease spectrum, biological characteristics, resistant mechanisms, and typing methods. Lebanese Abstracts 1996, B98, 1997. Barbour, E.K., Abi-Ghanem, D., Hamadeh, S., Eid, A., Talhouk, R. and Hilan, C.*, Characterization of non-specificity in herbal immunopotentiators of the cell-mediated and humoral immune systems of chickens. Lebanese Abstracts 1995, D109, 1995. Barbour, E.K., Dalloul, R., Talhouk, R., Hilan, C.*, Araj, G. and Hamadeh, S., Jamal, H., Virulence of predominant Salmonella serotypes recovered from chickens: Identification of their epidemiological markers in relation to human isolates. Lebanese Abstracts 1995, D111, 1997. Barbour, E.K., Kahoul, M., Hamadeh, S., Hilan, C.* and Talhouk, R., The role of serological profiling technology in poultry breeders. Lebanese Abstracts 1995, D113, 1995. Barbour, E.K., Hamadeh, S.K., Abi Ghanem, D., Haddad, J.J. and Safieh-Garabedian, B., Immunopotentiation in chicken layers by thymulin, thymopoietin and zinc. Forty-Sixth Western Poultry Conference, Sacramento, California, USA, 1997. Barbour, E.K., Hamadeh, S.K., Talhouk, R. and Hilan, C.*, Demands, facilities, and training needs for characterization of strains involved in livestock diseases of Lebanon. Eighth International Conference of Institutions of Tropical Veterinary Medicine, Berlin, Germany, 1995. Baydoun, E. and Brett, C.T.*, Distribution of xylosyltransferases within the Golgi apparatus in peas. Proceedings of the Seventh Cell Wall Meeting, Santiago de Compostela, Spain, 195, 1995. Brett, C.T.* and Baydoun, E., Mechanisms of xylan biosynthesis and insertion into the wall. Proceedings of the Seventh Cell Wall Meeting, Santiago de Compostela, Spain, 194, 1995. ———, Glucuronoxylan biosynthesis and insertion into the plant cell wall. Proceedings of the Plant Polysaccharides Symposium, Nantes, France, 55, 1996. Brett, C.T.*, McDougall, G.J.* and Baydoun, E., Xylan biosynthesis in pea and flax. Proceedings of the Sixth Annual Meeting of the Scottish Cell Wall Group, Edinburgh, UK, S10, 1996. ———, Xylan biosynthesis in differentiating xylem in flax and pea. Proceedings of the International Conference on Cellular and Molecular Aspects of Plant Cell Differentiation, Beirut, Lebanon, 24, 1996. Dagher, S., Emerging uses of food composition data. Meeting on Food Composition Activities in the Mediterranean Countries (MEFOODS), Paphos, Cyprus, 1997. El-Dana, R.A., Talhouk, R.S., Barbour, E.K. and Araj, G.F., Recovery and characterization of Campylobacter strains from human poultry in Lebanon. Lebanese Abstracts 1995, D18, 1995. Gali-Muhtasib, H.U., Ahmad, N.*, Jawed, S.*, Mukhtar, H.* and Agarwal, R.*, Inhibition of epidermal growth factor receptor (EGFr) tyrosine kinase activity by silymarin, a polyphenolic antioxidant and potent cancer chemopreventive agent. Proceedings of the Eighty-Eighth Annual Meeting of the American Association for Cancer Research, San Diego, California, USA, 38, 263, 1997. Gali-Muhtasib, H.U., Khatib, S., Perchellet, J.P.* and Samad, A., Inhibition of gallotannins and proanthocyanidins of the biochemical markers of skin tumor promotion by ultraviolet B radiation. Proceedings of the Eighty-Eighth Annual Meeting of the American Association for Cancer Research, San Diego, California, USA, 247, 1997. Hamadeh, S., Zoubiane, Z., Talhouk, R. and Barbour, E., Postpartum fertility and immune response in lactating Awassi and Finn X Texel X Awassi Ewes. Lebanese Abstracts 1995, D116, 1995. Jabbur, S.J., Kanaan, S.A., Safieh-Garabedian, B., Atweh, S.F. and Saade, N.E., Effects of various drugs on endotoxin (ET)-induced hyperalgesia in rodents. Twenty-Sixth Annual Meeting of the Society for Neuroscience, Washington, D.C. USA, 1812, 1996. Kanaan, S.A., Saade, N.E., Jabbur, S.J., Atweh, S.F. and Safieh-Garabedian, B., Interleukin (IL)-10 reduces significantly endotoxin (ET)-induced peripheral hyperalgesia in mice. Twenty-Sixth Annual Meeting of the Society for Neuroscience, Washington D.C., USA, 1812, 1996. Knio, K., Baydoun, E., Abou Fakhr,E., Malak, J. and Nuwayri-Salti, N., Isoenzyme characterization using starch gel electrophoresis of Leishmania isolates from Lebanon. Proceedings of the Forty-Fifth Annual Meeting of the American Society of Tropical Medicine and Hygiene, Baltimore, USA, 288, 1996. Kreydiyyeh, S., Epinephrine inhibits the mucosal transport of phenylalanine in rat enterocytes. Experimental Biology 97, New Orleans, USA, 1997. Masaa’d, M., Araj, G. F., Simaa’n, C. J. and Talhouk, R. S., Molecular typing of methicillin-resistant Staphylococcus aureus isolates from community individuals, patients, and hospital staff in Lebanon. Lebanese Abstracts 1996, D249, 1997. Perchellet, J.P.*, Perchellet, E.M.*, Gao, X.M.*, Gali-Muhtasib, H.U., Newell, S.W.*, Chen, G.* and Bottari, V.*, Characterization of the tumor-promoting activity of thapsigargin in SENCAR mouse skin and its modulation by gallotannin. Proceedings of the Eighty-Sixth Meeting of the American Association for Cancer Research, San Francisco, California, USA, 36, 123, 1995. Perchellet, J.P.*, Gao, X.M.*, Perchellet, E.M.*, Gali-Muhtasib, H.U., Rodriguez, L.* and Hemingway, R.W.*, Antitumor-promoting activity of loblolly pine bark condensed tannin in mouse epidermis in vivo. Seventeenth International Conference on Polyphenols, INRA Editions, Paris, 69, 407-408, 1995. Rizk, S., Baydoun, E. and Brett, C.T.*, Localization of methyltransferases within the Golgi apparatus. Proceedings of the Seventh Annual Meeting of the Scottish Cell Wall Group, 16-17, Abderdeen, U.K., 1997. Safieh-Garabedian, B., Jalakhian, R.H., Jabbur, S.J., Saade, N.E. and Kanaan, S.A. Thymulin reduces hyperalgesia by decreasing cytokine production. International Symposium on the Application of the Theory of Metabolic Regulation to Pain, Yerevan, Armenia, 1996. Safieh-Garabedian, B., Kanaan, S.A., Haddad, J.J., Malik, S., Abdelnoor, A., Saade, N.E. and Jabbur, S.J., Hyperalgesia produced by endotoxin (ET) injections: A new model for localized inflammtory pain. Society for Neuroscience Twenty-Fifth Annual Meeting, San Diego, California, USA, 1995. Safieh-Garabedian, B., Kanaan, S.A., Jabbur, S.J., Atweh, S.F. and Saade, N.E., Low doses of thymulin injection in the hindpaw of rats causes localized hyperalgesia. Society for Neuroscience Twenty-Sixth Annual Meeting, Washington, DC, USA, 1996. Safieh-Garabedian, B., Kanaan, S.A., Jabbur, S.J. and Saade, N.E., Low concentration of thymulin injection induces hyeralgesia in rats. Second Meeting of European Neuroscience, Strasbourg, France, 1996. Saleh, M.A., Zahed, L.F., Talhouk, R.S., The molcular basis of b -thalassaemia in Lebanon: A reassessment. Lebanese Abstracts 1995, C7, 1995. ———, The molecular basis of b -thalassaemia in Lebanon and its neighbouring countries. Lebanese Abstracts 1996, B100, 1997. Sympson, C.J.*, Alexander, C.*, Howard, C.*, Talhouk, R., Werb, Z.* and Bissell, M.*., Transgenic expression of the tissue inhibitor of metalloproteinase-1 (TIMP-1) in the mouse mammary gland delays involution and apoptosis. Molecular Biology of the Cell, 5, 428a, 1995. Talhouk, R.S., Abdo, R.A. and Saad, A., Lactose intolerance: Diagnosis and dietary treatment with milk substitutes. Lebanese Abstracts 1996, B99, 1997. Talhouk, R.S., El-Dana, R.A., Barbour, E.K. and Araj, G.F., Prevalence and characterization of Campylobacter in Lebanon (Abstract). American Society of Microbiology, 1997. Talhouk, R.S., Neiswander, R.L.* and Schanbacher, F.L.*, Developmental regulation and partial characterization of growth factors in the bovine mammary gland. Lebanese Abstracts 1996, A12, 1997. Talhouk, R.S., Simaa’n, C.J., Masaa’d, M.J., Matar, G. and Araj, G.F., Detection of “mec A” in meticillin-resistant Staphylococcus aureus by polymerase chain reaction. Lebanese Abstracts 1996, D248, 1997. Talhouk, R., Zoubiane, G. S., Hamadeh, S. K. and Barbour,
E., Characterization and developmental regulation of mammary-derived gelatinases
and growth promoting activities in sheep. Molecular Biology of the Cell,
6, 173a, 1995.
El-Dana, R.A., Recovery and characterization of Campylobacter strains from humans and poultry in Lebanon (1996). Advisor: R. Talhouk; co-advisor: G. Araj (Dept. of Pathology and Laboratory Medicine). Haddad, J., Hyperalgesia induced by local endotoxin injections and its modulation by various anti-inflammatory and analgesic drugs (1996). Advisor: S. A. Kanaan; co-advisor: N. Saade (Dept. of Human Morphology). Jalakhian, R., The effects of thymulin on the endotoxin-induced peripheral hyperalgesia (1996). Advisor: S. A. Kanaan; co-advisor: B. Safieh-Garabedian. Khatib, S. H., Inhibitory effects of plant tannins on the biochemical markers of skin tumor promotion induced by ultraviolet B radiation (1997). Advisor: H. Gali- Muhtasib. Masaad, M. J., Molecular typing of Methicillin-resistant Staphylococcus aureus isolates from community, individuals, patients and hospital staff in Lebanon (1996). Advisor: R. Talhouk; co-advisor: G. Araj (Dept. of Pathology and Laboratory Medicine). Saleh, M. A.-H., The molecular basis of b -Thallassaemia in Lebanon: A reassessment (1995). Advisor: R. Talhouk; co-advisor: L. Zahed (Dept. of Pathology and Laboratory Medicine). Tawk, R., Isoenzyme characterisation of Leishmania isolates from Lebanon (1997). Advisor: E. Baydoun.
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